|
KMID : 0378019740170070933
|
|
New Medical Journal
1974 Volume.17 No. 7 p.933 ~ p.939
|
|
|
Histochemical Study on Fibrous Elements and Mucopolysaccharides in the Human Developing Dentin
|
|
|
|
|
Abstract
|
|
|
It has been suggested that the fibrous elements and several organic components have important roles in the formation and the mineralization of dentin matrix.
This investigation dealed with the localization and interrelationship of Korff´s fiber and acid mucopolysaccharides with mineralization in the developing dentin histochemically.
The lower anterior regions were removed from 14 fetuses, ranging from 3 monthes to 6 monthes of age. They were fixed in 10% formalin, alcoholic formalin, Carnoy´s solution and etc.,
They were not decalcified and embedded in paraffin with vacuum pump.
The histological and histochemical staining methods used were silver impregnation method(Gomori), aniline blue stain(Mallory), peracetic acid-aldehyde fuchsin-Halmi method, periodic acid-paradiamine method (Spicer), colloidal iron-hematoxylin stain(Mowry), mixed diamine stain(Spicer), differentiation of cellular RNA and DNA stain(Swift), Van Gieson stain and hematoxylin-eosin stain.
The resutls were as follows:
1) Stain ability of argyrophilic Korff´s fibers were gradually reduced by mineralization of dentin matrix.
2) Peritubular matrix gave intense activity of acid mucosubstance and chiefly sulphated acid,mu￢copolysaccharide were pronounced.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|